Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays

Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of mic...

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Bibliografiske detaljer
Main Authors: Fierer, Noah, Jackson, Jason A., Vilgalys, Rytas, Jackson, Robert B.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2005
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1169028/
https://ncbi.nlm.nih.gov/pubmed/16000830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.7.4117-4120.2005
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