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Quantitative Detection of Legionella pneumophila in Water Samples by Immunomagnetic Purification and Real-Time PCR Amplification of the dotA Gene

A new real-time PCR assay was developed and validated in combination with an immunomagnetic separation system for the quantitative determination of Legionella pneumophila in water samples. Primers that amplify simultaneously an 80-bp fragment of the dotA gene from L. pneumophila and a recombinant fr...

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Hlavní autoři: Yáñez, M. A., Carrasco-Serrano, C., Barberá, V. M., Catalán, V.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1169023/
https://ncbi.nlm.nih.gov/pubmed/16000746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.7.3433-3441.2005
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