Analysis of Microbial Gene Transcripts in Environmental Samples

We analyzed gene expression in marine and freshwater bacterioplankton communities by the direct retrieval and analysis of microbial transcripts. Environmental mRNA, obtained from total RNA by subtractive hybridization of rRNA, was reverse transcribed, amplified with random primers, and cloned. Appro...

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Detalhes bibliográficos
Main Authors: Poretsky, Rachel S., Bano, Nasreen, Buchan, Alison, LeCleir, Gary, Kleikemper, Jutta, Pickering, Maria, Pate, Whitney M., Moran, Mary Ann, Hollibaugh, James T.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168992/
https://ncbi.nlm.nih.gov/pubmed/16000831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.7.4121-4126.2005
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