Cloning and Expression of Islandisin, a New Thermostable Subtilisin from Fervidobacterium islandicum, in Escherichia coli

A gene encoding a subtilisin-like protease, designated islandisin, from the extremely thermophilic bacterium Fervidobacterium islandicum (DSMZ 5733) was cloned and actively expressed in Escherichia coli. The gene was identified by PCR using degenerated primers based on conserved regions around two o...

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Detalhes bibliográficos
Main Authors: Gödde, Carolin, Sahm, Kerstin, Brouns, Stan J. J., Kluskens, Leon D., van der Oost, John, de Vos, Willem M., Antranikian, Garabed
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168981/
https://ncbi.nlm.nih.gov/pubmed/16000809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.7.3951-3958.2005
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