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The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-l-phenylalanyl-l-serine methyl ester

1. Purified stem bromelain (EC 3.4.22.4) was eluted from Sephadex G-100 as a single peak. The specific activity across the elution peak was approximately constant towards p-nitrophenyl hippurate but increased with elution volume with N(2)-benzoyl-l-arginine ethyl ester as substrate. 2. The apparent...

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Hlavní autor: Wharton, Christopher W.
Médium: Artigo
Jazyk:Inglês
Vydáno: 1974
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168426/
https://ncbi.nlm.nih.gov/pubmed/4462742
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