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Kinetic studies of oxidized nicotinamide–adenine dinucleotide-facilitated reactions of d-glyceraldehyde 3-phosphate dehydrogenase

The kinetics of the acylation of d-glyceraldehyde 3-phosphate dehydrogenase from pig muscle by 1,3-diphosphoglycerate in the presence of NAD(+) has been analysed by using the relaxation temperature-jump method. At pH7.2 and 8°C the rate of acylation of the NAD(+)-bound (or holo-) enzyme was 3.3×10(5...

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Detalhes bibliográficos
Main Authors: Harrigan, Patricia J., Trentham, David R.
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168390/
https://ncbi.nlm.nih.gov/pubmed/4376943
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