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The binding of oxidized and reduced nicotinamide–adenine dinucleotides to bovine liver uridine diphosphate glucose dehydrogenase

The binding of NAD(+) and NADH to bovine liver UDP-glucose dehydrogenase was studied by using gel-filtration and fluorescence-titration methods. The enzyme bound 0.5mol of NAD(+) and 2 mol of NADH/mol of subunit at saturating concentrations of both substrate and product. The dissociation constant fo...

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Detalhes bibliográficos
Main Authors: Gainey, Paul A., Phelps, Charles F.
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168171/
https://ncbi.nlm.nih.gov/pubmed/4377102
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