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Affinity chromatography of bovine trypsin. A rapid separation of bovine α- and β-trypsin
Affinity adsorbents for bovine trypsin were prepared by covalently coupling p-(p′-amino-phenoxypropoxy)benzamidine to cellulose and to agarose. Trypsin binds to both adsorbents at pH6–8 and is released at low pH values or in the presence of n-butylamine hydrochloride. Pure β-trypsin may be eluted fr...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1974
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1168110/ https://ncbi.nlm.nih.gov/pubmed/4477006 |
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