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Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes.

The oligonucleotide-directed RNase H sensitivity of a yeast (Saccharomyces cerevisiae) pre-mRNA was determined in an in vitro splicing reaction. While most of the pre-mRNA was sensitive to cleavage, the regions of the 5' splice site and TACTAAC box were found to be highly resistant. The biochem...

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Bibliografske podrobnosti
Main Authors: Rymond, B C, Rosbash, M
Format: Artigo
Jezik:Inglês
Izdano: 1986
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1167388/
https://ncbi.nlm.nih.gov/pubmed/2435546
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