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Accurate in vitro splicing of two pre-mRNA plant introns in a HeLa cell nuclear extract.
Two plant introns along with flanking exon sequences have been isolated from an amylase gene of wheat and a legumin gene of pea and cloned behind the phage SP6 promoter. Pre-mRNAs produced by in vitro transcription with SP6 RNA polymerase were tested for their ability to be spliced in a HeLa cell nu...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1986
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1167218/ https://ncbi.nlm.nih.gov/pubmed/2431897 |
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