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Purification and subunit analysis of wheat-germ ribonucleic acid polymerase II

A procedure is described for the purification of the α-amanitin-sensitive DNA-dependent RNA polymerase [EC 2.7.7.6] from wheat germ. Solubilization of the enzyme activity was achieved by sonication of a crude extract in a high-salt buffer. Purification involved precipitation with protamine sulphate...

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Bibliografske podrobnosti
Main Authors: Jendrisak, Jerome J., Becker, Wayne M.
Format: Artigo
Jezik:Inglês
Izdano: 1974
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1166341/
https://ncbi.nlm.nih.gov/pubmed/4853970
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