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Purification of normal human urinary N-acetyl-beta-hexosaminidase A by affinity chromatography.

N-Acetyl-beta-hexosaminidase A was purified 1000-fold from human urine by chromatography on DEAE-Sephadex followed by concanavalin A--Sepharose affinity chromatography. The optimal pH range was 4.4--4.5 for both the N-acetylglucosamine and N-acetylgalactosamine derivatives. The Km values were 0.51 m...

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Hlavní autoři: Banerjee, D K, Basu, D
Médium: Artigo
Jazyk:Inglês
Vydáno: 1975
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1165192/
https://ncbi.nlm.nih.gov/pubmed/242317
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