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Purification and properties of rat brain pyruvate carboxylase.

Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few su...

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Detalhes bibliográficos
Main Authors: Mahan, D E, Mushahwar, I K, Koeppe, R E
Formato: Artigo
Idioma:Inglês
Publicado em: 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1165183/
https://ncbi.nlm.nih.gov/pubmed/1238083
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