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Alloxan cytotoxicity in vitro. Microscope photometric analyses of Trypan Blue uptake by pancreatic islet cells in suspension.

Suspensions of islet cells were prepared by shaking pancreatic islets from non-inbred ob/ob mice in a Ca2+-free buffer. The cells were incubated with or without 20 mM-alloxan, and subsequently with Trypan Blue. The uptake of Trypan Blue by cell nuclei was analysed by microscope photometry and by cou...

詳細記述

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書誌詳細
主要な著者: Grankvist, K, Lernmark, A, Täljedal, I B
フォーマット: Artigo
言語:Inglês
出版事項: 1977
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1164564/
https://ncbi.nlm.nih.gov/pubmed/322658
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