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4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain.

1. 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (Nbd chloride) was used as a reactivity probe to characterize the active centres of papin (EC 3.4.22.2), ficin (EC 3.4.22.3) and bromelain (EC 3.4.22.4). 2. In the pH range 0-8 Nbd chloride probably exists mainly as a monocation, possibly with the proton lo...

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Bibliografske podrobnosti
Main Authors: Shipton, M, Stuchbury, T, Brocklehurst, K
Format: Artigo
Jezik:Inglês
Izdano: 1976
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1164110/
https://ncbi.nlm.nih.gov/pubmed/11778
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