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The kinetics of rabbit muscle pyruvate kinase. Initial-velocity, substrate- and product-inhibition and isotopic-exchange studies of the reverse reaction.

1. An assay, based on the transfer of label from [gamma-32P]ATP to [32P]phosphoenolpyruvate, suitable for a steady-state kinetic analysis of pyruvate kinase in the reverse direction (i.e. phosphoenolpyruvate synthesis), is described. 2. This assay was used in a kinetic investigation of the rabbit mu...

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Main Authors: Giles, I G, Poat, P C, Munday, K A
Format: Artigo
Jezik:Inglês
Izdano: 1976
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1163899/
https://ncbi.nlm.nih.gov/pubmed/985404
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