Solubilization of the ileal receptor for intrinsic factor--vitamin B-12 complex by digestion with papain.

Brush-border-membrane vesicles isolated from hamster ileum were incubated with either papain or Pronase P and subsequently centrifuged to obtain soluble (supernatant) and insoluble (pellet) fractions. Papain (4 units/ml) solubilized 95--100% of the sucrase and leucine naphthylamide-hydrolysing activ...

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Bibliographic Details
Main Author: Beesley, R C
Format: Artigo
Language:Inglês
Published: 1981
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1163528/
https://ncbi.nlm.nih.gov/pubmed/6280680
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