Sequencing along DNA fragments cloned in bacteriophage M13 by using internal primers. The sequence analysis of a yeast DNA fragment containing a replication origin

In the `shotgun' procedure for sequencing DNA, DNA fragments are cloned into a phage M13 vector and sequenced by using a flanking primer. In a variation of this procedure a longer DNA sequence is cloned into M13, the two single-stranded recombinants identified and sequenced by using a set of in...

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Bibliographic Details
Main Authors: Hindley, John, Phear, Geraldine A.
Format: Artigo
Language:Inglês
Published: 1981
Subjects:
Gene Structure and Function
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1163441/
https://ncbi.nlm.nih.gov/pubmed/6280678
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