Hydrophobic-interaction chromatography and anion-exchange chromatography in the presence of acetonitrile. A two-step purification method for human prolactin

Described is a two-chromatographic-step preparative-scale technique for the purification of human prolactin from a frozen pituitary homogenate. The method utilizes hydrophobic interaction chromatography on the mildly hydrophobic adsorbent phenyl-Sepharose CL-4B and anion-exchange chromatography on D...

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Bibliographic Details
Main Authors: Hodgkinson, Steven C., Lowry, Philip J.
Format: Artigo
Language:Inglês
Published: 1981
Subjects:
Peptide and Protein Structure
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1163418/
https://ncbi.nlm.nih.gov/pubmed/7340823
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