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Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of 'free' iron in biological systems by using bleomycin-dependent degradation of DNA.

Bleomycin in the presence of iron(II) degrades DNA to form a thiobarbituric acid-reactive product. This has been made the basis of a specific assay method for 'free' iron in biological fluids. Human synovial fluid, human cerebrospinal fluid and rat pleural-exudate fluid were found to conta...

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Autors principals: Gutteridge, J M, Rowley, D A, Halliwell, B
Format: Artigo
Idioma:Inglês
Publicat: 1981
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1163361/
https://ncbi.nlm.nih.gov/pubmed/6175315
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