Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reactivity probes.

Gelijkaardige items

• Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe.
  door: Malthouse, J P, et al.
  Gepubliceerd in: (1976)
• Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe.
  door: Brocklehurst, K, et al.
  Gepubliceerd in: (1979)
• The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain.
  door: Brocklehurst, K, et al.
  Gepubliceerd in: (1989)
• Covalent chromatography. Preparation of fully active papain from dried papaya latex
  door: Brocklehurst, Keith, et al.
  Gepubliceerd in: (1973)
• Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe.
  door: Brocklehurst, K, et al.
  Gepubliceerd in: (1981)