Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reactivity probes.

פריטים דומים

• Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe.
  מאת: Malthouse, J P, et al.
  יצא לאור: (1976)
• Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe.
  מאת: Brocklehurst, K, et al.
  יצא לאור: (1979)
• The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain.
  מאת: Brocklehurst, K, et al.
  יצא לאור: (1989)
• Covalent chromatography. Preparation of fully active papain from dried papaya latex
  מאת: Brocklehurst, Keith, et al.
  יצא לאור: (1973)
• Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe.
  מאת: Brocklehurst, K, et al.
  יצא לאור: (1981)