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Calmodulin-free skeletal-muscle troponin C prepared in the absence of urea.

A method is described for the rapid preparation of electrophoretically pure troponin C from rabbit skeletal-muscle myofibrils that avoids the use of urea. The three-step procedure includes extraction od the myofibrils with EDTA-containing buffers, one-step elution from DEAE-Sephadex and Sephadex G-1...

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Detalles Bibliográficos
Main Authors: Cox, J A, Comte, M, Stein, E A
Formato: Artigo
Idioma:Inglês
Publicado: 1981
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1162873/
https://ncbi.nlm.nih.gov/pubmed/6272746
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