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Calcium-binding constants of trypsin and trypsinogen. A reassessment.

The Ca2+-binding constants for trypsin and trypsinogen have been reassessed by using enzyme that has been purified by affinity chromatography and measuring the distribution of 45Ca2+ between the protein and a cation exchanger. The pKCa2+ value of 4.5 for the high-affinity site on trypsin was 1 logar...

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Autors principals: Cliffe, S G, Grant, D A
Format: Artigo
Idioma:Inglês
Publicat: 1981
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1162647/
https://ncbi.nlm.nih.gov/pubmed/7305950
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