A rapid method for the isolation of ribonuclease from yeast (Saccharomyces carlsbergensis).

A ribonuclease (RNAase; EC 3.1.14.1) from brewer's yeast was purified 90-fold. Crude RNAase was initially separated from other proteins by precipitation at pH 4.0 after incubation of the mechanically disrupted yeast cells at pH 6.0 and 52 degrees C for 30 min. The RNAase was purified from the s...

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Bibliografiske detaljer
Main Authors: Shetty, J K, Weaver, R C, Kinsella, J E
Format: Artigo
Sprog:Inglês
Udgivet: 1980
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1162006/
https://ncbi.nlm.nih.gov/pubmed/7006598
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