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Isolation of lysyl hydroxylase, an enzyme of collagen synthesis, from chick embryos as a homogeneous protein.

Two procedures are reported for the purification of lysyl hydroxylase, both procedures involving (NH4)2SO4 fractionation, affinity chromatography on concanavalin A-agarose and elution of the column with ethylene glycol. The additional steps in procedure A consist of gel filtration and chromatography...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Turpeenniemi-Hujanen, T M, Puistola, U, Kivirikko, K I
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1980
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1161995/
https://ncbi.nlm.nih.gov/pubmed/6779811
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