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Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreased by reagent protonation.

The active centres of chymopapains A and B (jointly designated EC 3.4.22.6) and papaya (Carica papaya L.) peptidase A were investigated by using 2,2'-dipyridyl disulphide and 5,5'-dithiobis-(2-nitrobenzoic acid) as thiol-specific reactivity probes. Whereas the first active-centre pKa value...

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Détails bibliographiques
Auteurs principaux: Brocklehurst, K, Baines, B S, Mushiri, M S
Format: Artigo
Langue:Inglês
Publié: 1980
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1161932/
https://ncbi.nlm.nih.gov/pubmed/7006597
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