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Subcellular distribution of enzymes determined by rapid digitonin fractionation of isolated hepatocytes

Conditions were determined for rapid separation of cytosolic and mitochondrial compartments by digitonin fractionation of rat hepatocytes. The minimum time required for separation of mitochondrial and cytosolic enzyme markers decreased rapidly with increasing temperature. Kyro EOB, a non-ionic deter...

詳細記述

保存先:
書誌詳細
主要な著者: Janski, Alvin M., Cornell, Neal W.
フォーマット: Artigo
言語:Inglês
出版事項: 1980
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1161592/
https://ncbi.nlm.nih.gov/pubmed/7378059
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