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The reversible immobilization of proteins on nylon activated through the formation of a substituted imidoester, and its unusual properties.
Alkylation of nylon produces nylon imidate, which is used for the covalent coupling of enzymes and other proteins and ligands. Nylon imidate is unusually stable when stored wet, with a half-life of about 60 days. Reaction with enzymes has an optimum about pH 7-8 and is extremely rapid, with about 60...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1161575/ https://ncbi.nlm.nih.gov/pubmed/534505 |
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