Tissue-culture fractionation. Zonal centrifugation of Lettrée cells homogenized by glycerol-induced lysis: subfractionation of membranes in sucrose gradients.

1. Lettrée cells were grown intraperitoneally in MF-1 mice. 2. Cells that were loaded with glycerol were swollen in 0.1 M-sucrose and disrupted by Dounce homogenization. 3. Early-passage Lettrée cells were more easily disrupted than late-passage cells by this method, and the former produced larger f...

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Opis bibliograficzny
Główni autorzy: Graham, J M, Coffey, K H
Format: Artigo
Język:Inglês
Wydane: 1979
Hasła przedmiotowe:
Dostęp online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1161245/
https://ncbi.nlm.nih.gov/pubmed/496904
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https://ncbi.nlm.nih.gov/pmc/articles/PMC1161245/
https://ncbi.nlm.nih.gov/pubmed/496904