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Cross-linking and (1)H n.m.r. spectroscopy of the pyruvate dehydrogenase complex of Escherichia coli

The pyruvate dehydrogenase complex of Escherichia coli was treated with o-phenylene bismaleimide in the presence of the substrate pyruvate, producing almost complete cross-linking of the lipoate acetyltransferase polypeptide chains as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophore...

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Detalhes bibliográficos
Main Authors: Packman, Leonard C., Perham, Richard N., Roberts, Gordon C. K.
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1158492/
https://ncbi.nlm.nih.gov/pubmed/6753833
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