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A method for determining kinetic parameters at high enzyme concentrations.

A graphical method is described which allows determination of kinetic parameters when substrate, inhibitor or activator concentrations must be in the vicinity of the enzyme concentration and a significant fraction of ligand is bound. Velocity is measured at several ligand: enzyme ratios at two or mo...

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Bibliographic Details
Main Authors: Halfman, C J, Marcus, F
Format: Artigo
Language:Inglês
Published: 1982
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1158230/
https://ncbi.nlm.nih.gov/pubmed/6285896
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