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Purification and characterization of the major aminopeptidase from human skeletal muscle.

The major aminopeptidase from human quadriceps muscle was purified (as judged by polyacrylamide-gel electrophoresis) by anion-exchange chromatography (two steps) and gel filtration (two steps). The enzyme showed maximum activity at pH 7.3, in the presence of 1 mM-2-mercaptoethanol and 0.5 mM-Ca2+ io...

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Detalhes bibliográficos
Main Authors: Mantle, D, Hardy, M F, Lauffart, B, McDermott, J R, Smith, A I, Pennington, R J
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1154401/
https://ncbi.nlm.nih.gov/pubmed/6882360
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