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An efficient method for the isolation and separation of basolateral-membrane and luminal-membrane vesicles from rabbit kidney cortex.

A procedure for isolation and separation of purified luminal-membrane and basolateral-membrane vesicles from adult and newborn rabbit renal cortex by using Ca2+/Mg2+ precipitation, differential centrifugation and a self-orienting Percoll-gradient centrifugation is described. The purity of the membra...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sheikh, M I, Kragh-Hansen, U, Jørgensen, K E, Røigaard-Petersen, H
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1982
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1153973/
https://ncbi.nlm.nih.gov/pubmed/7159406
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