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Enzymic determination of inorganic phosphates, organic phosphates and phosphate-liberating enzymes by use of nucleoside phosphorylase-xanthine oxidase (dehydrogenase)-coupled reactions.

Coupled enzyme assays are described for measuring inorganic phosphates, organic phosphates and phosphate-liberating enzymes in biological material. The assays all determine Pi by its reaction with inosine, catalysed by nucleoside phosphorylase; this yields ribose 1-phosphate and hypoxanthine. The hy...

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Bibliografiske detaljer
Main Authors: de Groot, H, Noll, T
Format: Artigo
Sprog:Inglês
Udgivet: 1985
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1152609/
https://ncbi.nlm.nih.gov/pubmed/2996493
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