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Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp(uv)) from Escherichia coli
BACKGROUND: Transformed cells of Escherichia coli DH5-α with pGFPuv, induced by IPTG (isopropyl-β-d-thiogalactopyranoside), express the green fluorescent protein (gfp(uv)) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followe...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2002
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC115201/ https://ncbi.nlm.nih.gov/pubmed/11972900 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-2-7 |
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