Use of the Genomic Subtractive Hybridization Technique To Develop a Real-Time PCR Assay for Quantitative Detection of Prevotella spp. in Oral Biofilm Samples

Genomic subtractive hybridization was used to design Prevotella nigrescens-specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hy...

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Bibliographische Detailangaben
Hauptverfasser: Nagashima, Shiori, Yoshida, Akihiro, Suzuki, Nao, Ansai, Toshihiro, Takehara, Tadamichi
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Bacteriology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151961/
https://ncbi.nlm.nih.gov/pubmed/15956428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.6.2948-2951.2005
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