Mapping dispersed repetitive loci using semi-specific PCR cloning and somatic cell hybrid mapping

A simple and effective method based upon semi-specific PCR followed by cloning has been developed. Chromosomal mapping of the generated fragment on a somatic cell hybrid panel identifies the chromosomal position, and yields a unique sequence tag for the site. Using this method, the chromosomal locat...

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Bibliographic Details
Main Authors: Deng, Yi-Mo, Lee, Jun-Heon, Moran, Chris, Jin, Jun-Hua, Tuch, Bernard E., Rawlinson, William D.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2000
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC115187/
https://ncbi.nlm.nih.gov/pubmed/11095699
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