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Synthesis of a gene for rat liver fatty-acid-binding protein and its expression in Escherichia coli.

A gene coding for rat liver fatty-acid-binding protein (FABP) has been constructed by the single-step ligation of ten synthetic oligonucleotides. The gene has been cloned into the bacterial expression vector pKK223-3. Induction of protein synthesis from this gene results in over expression of an FAB...

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Detalhes bibliográficos
Main Authors: Worrall, A F, Evans, C, Wilton, D C
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151350/
https://ncbi.nlm.nih.gov/pubmed/1898328
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