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Gene synthesis, expression in Escherichia coli, purification and characterization of the recombinant bovine acyl-CoA-binding protein.

A synthetic gene encoding the 86 amino acid residues of mature acyl-CoA-binding protein (ACBP), and the initiating methionine was constructed. The synthetic gene was assembled from eight partially overlapping oligonucleotides. Codon usage and nucleotides surrounding the ATG translation-initiation co...

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Detalles Bibliográficos
Main Authors: Mandrup, S, Højrup, P, Kristiansen, K, Knudsen, J
Formato: Artigo
Idioma:Inglês
Publicado: 1991
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151077/
https://ncbi.nlm.nih.gov/pubmed/2064616
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