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Inhibition of the specific binding of human lactotransferrin to human peripheral-blood phytohaemagglutinin-stimulated lymphocytes by fluorescein labelling and location of the binding site.

Labelling of human lactotransferrin with fluorescein 5'-isothiocyanate (FITC) in an equimolar ratio inhibits the binding of the protein to phytohaemagglutinin-activated human peripheral-blood lymphocytes. Therefore it can be assumed that FITC reacts at, or near, the receptor-binding site. Three...

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Autores principales: Legrand, D, Mazurier, J, Maes, P, Rochard, E, Montreuil, J, Spik, G
Formato: Artigo
Lenguaje:Inglês
Publicado: 1991
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151065/
https://ncbi.nlm.nih.gov/pubmed/1648350
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