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Redox properties and cross-linking of the dithiol/disulphide active sites of mammalian protein disulphide-isomerase.

1. The redox properties of the active-site dithiol/disulphide groups of PDI were determined by equilibrating the enzyme with an excess of GSH + GSSG, rapidly alkylating the dithiol form of the enzyme to inactivate it irreversibly, and determining the proportion of the disulphide form by measuring th...

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Detalhes bibliográficos
Main Authors: Hawkins, H C, de Nardi, M, Freedman, R B
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1150058/
https://ncbi.nlm.nih.gov/pubmed/2025221
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