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Proteolysis of Monomeric Recombinant Rotavirus VP4 Yields an Oligomeric VP5* Core

Rotavirus particles are activated for cell entry by trypsin cleavage of the outer capsid spike protein, VP4, into a hemagglutinin, VP8*, and a membrane penetration protein, VP5*. We have purified rhesus rotavirus VP4, expressed in baculovirus-infected insect cells. Purified VP4 is a soluble, elongat...

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Detalhes bibliográficos
Main Authors: Dormitzer, Philip R., Greenberg, Harry B., Harrison, Stephen C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC114969/
https://ncbi.nlm.nih.gov/pubmed/11462006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.75.16.7339-7350.2001
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