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Vacuolar H(+)-ATPase of adrenal secretory granules. Rapid partial purification and reconstitution into proteoliposomes.
A procedure has been developed for the rapid purification and reconstitution into phospholipid vesicles of the proton-translocating ATPase of bovine adrenal chromaffin-granule membranes. It involves fractionation of the membranes with Triton X-114, resolubilization of the ATPase with n-octyl glucosi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1149522/ https://ncbi.nlm.nih.gov/pubmed/2171495 |
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