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Purification and characterization of a tyrosine-specific protein kinase of Mr 60,000 and comparison with a kinase of Mr 56,000 from rat spleen.

A tyrosine-specific protein kinase of Mr 60,000 (TK-I) was purified to near homogeneity from the particulate fraction of rat spleen. The purification procedure involved sequential chromatography of the detergent-solubilized enzyme on DEAE-Sephacel and hydroxyapatite columns. Polyacrylamide-gel elect...

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Detalhes bibliográficos
Main Authors: Swarup, G, Subrahmanyam, G, Rema, V
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1149039/
https://ncbi.nlm.nih.gov/pubmed/3401218
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