Electric shock-mediated transfection of cells. Characterization and optimization of electrical parameters.

The effect of various parameters on the electric shock-mediated permeabilization and transfection of CHO cells has been investigated. Up to 70% of the cells can be maintained transiently permeable to erythrosin B for periods of at least 1 h at 20 degrees C. Electrical conditions optimal for transien...

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Bibliographic Details
Main Authors: Winterbourne, D J, Thomas, S, Hermon-Taylor, J, Hussain, I, Johnstone, A P
Format: Artigo
Language:Inglês
Published: 1988
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1149020/
https://ncbi.nlm.nih.gov/pubmed/3401216
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