Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe.

1. The pH-dependences of the second-order rate constant (k) for the reactions of papain (EC 3.4.22.2) with 2-(acetamido)ethyl 2'-pyridyl disulphide and with ethyl 2-pyridyl disulphide and of k for the reaction of benzimidazol-2-ylmethanethiol (as a minimal model of cysteine proteinase catalytic...

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Bibliographic Details
Main Authors: Brocklehurst, K, Kowlessur, D, Patel, G, Templeton, W, Quigley, K, Thomas, E W, Wharton, C W, Willenbrock, F, Szawelski, R J
Format: Artigo
Language:Inglês
Published: 1988
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148922/
https://ncbi.nlm.nih.gov/pubmed/2839145
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