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Studies on the biotin-binding site of avidin. Tryptophan residues involved in the active site.

Egg-white avidin was modified with the tryptophan-specific reagent 2-hydroxy-5-nitrobenzyl bromide. The complete loss of biotin-binding activity was achieved upon modification of an average of one tryptophan residue per avidin subunit. The identity of the modified residues was determined by isolatin...

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Dettagli Bibliografici
Autori principali: Gitlin, G, Bayer, E A, Wilchek, M
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1988
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148846/
https://ncbi.nlm.nih.gov/pubmed/3355517
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