Suppression of fluorescence of tryptophan residues in proteins by replacement with 4-fluorotryptophan.

The tryptophan-auxotrophic Bacillus subtilis LC33 mutant strain utilizes either tryptophan or 4-fluorotryptophan for growth. Proteins therefore could be isolated from these cells in either tryptophan-containing or 4-fluorotryptophan-containing forms. Since 4-fluorotryptophan is non-fluorescent, tryp...

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Bibliografiske detaljer
Main Authors: Bronskill, P M, Wong, J T
Format: Artigo
Sprog:Inglês
Udgivet: 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148699/
https://ncbi.nlm.nih.gov/pubmed/3124823
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