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Preservation of the activity state of hepatic branched-chain 2-oxo acid dehydrogenase during the isolation of mitochondria.

A comparison was conducted of current methods for estimation of the activity states (proportion of enzyme in active, dephosphorylated, form) of hepatic branched-chain 2-oxo acid dehydrogenase. Practically all of the enzyme was active in freeze-clamped liver obtained from chow-fed and 48 h-starved ra...

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Autors principals: Zhang, B, Paxton, R, Goodwin, G W, Shimomura, Y, Harris, R A
Format: Artigo
Idioma:Inglês
Publicat: 1987
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148326/
https://ncbi.nlm.nih.gov/pubmed/3689325
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