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Purification, characterization and cellular localization of 5'-nucleotidase from Torpedo electric organ.

5'-Nucleotidase was isolated from the electric organ of the electric ray Torpedo marmorata after solubilization in Triton X-100 and deoxycholate by affinity chromatography on concanavalin A-Sepharose and AMP-Sepharose. The purified enzyme has a Km for AMP of 38 microM, with a maximal velocity o...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Grondal, E J, Zimmermann, H
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1987
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148201/
https://ncbi.nlm.nih.gov/pubmed/2822031
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